产品描述：TMB ELISA Substrate detects horseradish peroxidase (HRP) activity.产品形式：Liquid 使用指导：(a) After incubation with horseradish peroxidase (HRP) conjugated secondary antibody, wash plates with PBS/0.05% Tween-20. (b) Add 100 μl of TMB solution/well. Incubate for a specific amount of time or until desired signal is achieved. (c) Add 100 μl of ELISA Stop Solution. (d) Read at Absorbance 450 nm.组分：TMB is a one component system that is ready to use from the bottle.储存效期：Store at 4 °C in dark for 1 year, do not freeze.